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Photocatalytic disinfection treatments: viability, cultivability and metabolic changes of e. coli using diferent mesurements methods

Sanabria Gómez, Irma Janeth and Wist, Julien and Pulgarín Gómez, César (2011) Photocatalytic disinfection treatments: viability, cultivability and metabolic changes of e. coli using diferent mesurements methods. Dyna; Vol. 78, núm. 166 (2011); 150-157 DYNA; Vol. 78, núm. 166 (2011); 150-157 2346-2183 0012-7353 .

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URL oficial: http://revistas.unal.edu.co/index.php/dyna/article...

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In this work, the oxidative stress was produced by the illumination of either fixed TiO₂ or suspended TiO₂, and the relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) and Fluorescence In Situ Hybridisation (FISH) methods. Under certain oxidative conditions simulating incomplete treatments, viable cells that had lost their cultivability were observed to recover all of their physiological functions when transferred to fresh nutrient media. Moreover, alterations in the nutrient assimilation processes were studied by 1H-NMR during and after the photocatalytic treatment. This allowed us to shed some light on how the damage chain propagates during the treatment. This work demonstrate that the Standard Plate Count (SPC) method commonly used to estimate the c ell populations during disinfection processes is ill adapted, whereas the FISH-DVC pair offers a reliable alternative.

Tipo de documento:Artículo - Article
Información adicional:Derechos de autor reservados
Palabras clave:Photocatalysis, drinking water, E. coli, oxidative stress, titanium dioxide, cultivability, disinfection, NMR
Unidad administrativa:Revistas electrónicas UN > Dyna
Código ID:27832
Enviado por : Dirección Nacional de Bibliotecas STECNICO
Enviado el día :27 Junio 2014 19:02
Ultima modificación:01 Julio 2018 18:11
Ultima modificación:01 Julio 2018 18:11
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